A Simple Key For thr77 Unveiled
A Simple Key For thr77 Unveiled
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been observed in many tumors, which leads to chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a vital purpose in endorsing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already described. Two extra splice variants were claimed but in depth nucleotide sequence info is not really however out there.
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Blocking peptides are peptides that bind particularly on the focus on antibody and block antibody binding. These peptide normally incorporates the epitope identified via the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This information outlines further feasible optimization of mobile and immunoassay parameters to ensure the best possible outcomes are...
Antibodies sure to the blocking peptide no more bind towards the epitope on the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody by yourself, you can see which staining is certain.
Concentrate on Information and facts Cyclin E belongs to the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance throughout the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which lead to your temporal coordination of each mitotic party. Cyclin E varieties a fancy with and features as a regulatory subunit of CDK2, whose action is required for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is also degraded as cells progress by S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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